Round 7: Tossup 11

A formula containing the constants 5500, 1490, and 125 is used to calculate the extinction coefficient of these molecules, which is usually between 4 and 24. Potassium sodium tartrate is used to chelate (“KEE-late”) metal ions in a test for these molecules that gives a peak at 540 nanometers. These molecules are oxidized by copper-two-plus, and the resulting (10[1])copper-plus is reacted with a mixture of phosphomolybdate and phosphotungstate called the Folin–Ciocalteu (-5[1])(“fo-LEEN cho-call-TEH-oo”) reagent, (10[1])in an assay for quantifying these molecules. (10[1])These molecules are quantified by their absorption at 280 nanometers (10[3])in spectrophotometry. (10[2]-5[2])Coomassie (10[1])blue is used (-5[1])in the Bradford assay (10[1])to quantify (10[1])these molecules. (10[1])These molecules (10[1])are linearized in beta-mercaptoethanol (10[1])and SDS before being loaded (10[1])into a polyacrylamide gel. For 10 points, name these macromolecules composed of amino acids. (10[4])■END■ (10[2])

ANSWER: proteins [or polypeptides; accept amino acids or aromatic amino acids until read; prompt on phenols by asking “what larger molecules are those phenolic groups typically part of in that assay?”]
<Editors, Chemistry> | G. Prelims 7 - Georgia Tech A + Berkeley A + Bruin + Georgetown
= Average correct buzzpoint

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